APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-2μg/ml for 30 min at RT |
| Assay dependent |
|
Note :
IHC-P
Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
|
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant human full-length STAT3 protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ADMIO , ADMIO1 , APRF , HIES , STAT3 , signal transducer and activator of transcription 3
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]
Database
Research Area
DATA IMAGES
![Anti-STAT3 antibody [STAT3/2409] used in Protein Array (Protein Array). GTX18120](/templates/default/js/lightbox/images/loading.gif "Anti-STAT3 antibody [STAT3/2409] used in Protein Array (Protein Array). GTX18120")
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GTX18120 Protein Array Image
Analysis of Protein Array containing more than 19,000 full-length human proteins using STAT3 Mouse Monoclonal Antibody (STAT3/2409). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
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GTX18120 IHC-P Image
IHC-P analysis of human renal cell carcinoma tissue using GTX18120 STAT3 antibody [STAT3/2409].
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REFERENCE
There are currently no references for STAT3 antibody [STAT3/2409] (GTX18120). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for STAT3 antibody [STAT3/2409] (GTX18120). Be the first to share your experience with this product.
![Anti-STAT3 antibody [STAT3/2409] used in Protein Array (Protein Array). GTX18120](/upload/website/prouct_img/small/GTX18120/GTX18120_20200115_Protein-Array_2097_w_23060620_649.webp "Anti-STAT3 antibody [STAT3/2409] used in Protein Array (Protein Array). GTX18120")
![Anti-STAT3 antibody [STAT3/2409] used in IHC (Paraffin sections) (IHC-P). GTX18120](/upload/website/prouct_img/small/GTX18120/GTX18120_20200115_IHC-P_1086_w_23060620_635.webp "Anti-STAT3 antibody [STAT3/2409] used in IHC (Paraffin sections) (IHC-P). GTX18120")
![Anti-STAT3 antibody [STAT3/2409] used in Protein Array (Protein Array). GTX18120](/upload/website/prouct_img/normal/GTX18120/GTX18120_20200115_Protein-Array_2097_w_23060620_649.webp "Anti-STAT3 antibody [STAT3/2409] used in Protein Array (Protein Array). GTX18120")
![Anti-STAT3 antibody [STAT3/2409] used in IHC (Paraffin sections) (IHC-P). GTX18120](/upload/website/prouct_img/normal/GTX18120/GTX18120_20200115_IHC-P_1086_w_23060620_635.webp "Anti-STAT3 antibody [STAT3/2409] used in IHC (Paraffin sections) (IHC-P). GTX18120")